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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Cdk6 overexpression 293T lysate (whole cell) (ab94291)

  • Datasheet
  • SDS
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SDS-PAGE - Cdk6 overexpression 293T lysate (whole cell) (ab94291)
  • Western blot - Cdk6 overexpression 293T lysate (whole cell) (ab94291)

Overview

  • Product name

    Cdk6 overexpression 293T lysate (whole cell)
    See all Cdk6 lysates
  • General notes

    ab94291 is a 293T cell transfected lysate in which Human Cdk6 has been transiently over-expressed using a pCMV-Cdk6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Cdks
  • Background

    Function: Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Associated products

  • Corresponding Antibody

    • Anti-Cdk6 antibody (ab54576)

Applications

Our Abpromise guarantee covers the use of ab94291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • SDS-PAGE - Cdk6 overexpression 293T lysate (whole cell) (ab94291)
    SDS-PAGE - Cdk6 overexpression 293T lysate (whole cell) (ab94291)
    ab94291 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - Cdk6 overexpression 293T lysate (whole cell) (ab94291)
    Western blot - Cdk6 overexpression 293T lysate (whole cell) (ab94291)
    All lanes : Anti-Cdk6 antibody (ab54576) at 1/500 dilution

    Lane 1 : Cdk6 overexpression 293T lysate (whole cell) (ab94291)
    Lane 2 : 293T non-transfected lysate



    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Protocols

  • Transfected Lysate Preparation Notes

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab94291? Please let us know so that we can cite the reference in this datasheet.

    ab94291 has not yet been referenced specifically in any publications.

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