Overview

  • Product name

    Anti-Cdk6 (phospho Y13) antibody
    See all Cdk6 primary antibodies
  • Description

    Rabbit polyclonal to Cdk6 (phospho Y13)
  • Host species

    Rabbit
  • Specificity

    ab131439 detects endogenous levels of Cdk6 only when phosphorylated at tyrosine 13.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Tyrosine 13 (

    Q-Q-Y(p)-E-C

    ) of Human Cdk6 (NP_001138778.1).

  • Positive control

    • 293 cell extract treated with Hydroxyurea; HeLa cells; Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-Cdk6 (phospho Y13) antibody (ab131439) at 1/500 dilution

    Lane 1 : 293 cell extract untreated
    Lane 2 : 293 cell extract treated with Hydroxyurea

    Predicted band size: 37 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cdk6 (phospho Y13) with ab131439 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y13) with ab131439 at 1/100 dilution.

References

This product has been referenced in:

  • Liu Q  et al. Vascular endothelial growth receptor 1 acts as a stress-associated protein in the therapeutic response to thalidomide. Exp Ther Med 14:4263-4271 (2017). Read more (PubMed: 29075340) »
See 1 Publication for this product

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