Overview

  • Product name

    Anti-Cdk6 (phospho Y24) antibody
    See all Cdk6 primary antibodies
  • Description

    Rabbit polyclonal to Cdk6 (phospho Y24)
  • Host species

    Rabbit
  • Specificity

    ab131469 detects Cdk6 only when phosphorylated at tyrosine 24.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Cdk6.
    Sequence:

    G-A-Y(p)-G-K

  • Positive control

    • Human colon carcinoma tissue; extracts from 293 cells; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab131469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-Cdk6 (phospho Y24) antibody (ab131469) at 1/500 dilution

    Lane 1 : Extract from 293 cells; untreated
    Lane 2 : Extract from 293 cells; treated with starvation

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling Cdk6 (phospho Y24) with ab131469 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling Cdk6 (phospho Y24) with ab131469 at 1/50 dilution. The image on the right is preincubated with blocking peptide.

References

This product has been referenced in:

  • Wang M  et al. Long non-coding RNA OIP5-AS1 promotes proliferation of lung cancer cells and leads to poor prognosis by targeting miR-378a-3p. Thorac Cancer 9:939-949 (2018). Read more (PubMed: 29897167) »
  • Adam MG  et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle 14:3734-47 (2015). WB . Read more (PubMed: 26654769) »
See all 2 Publications for this product

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