Product nameAnti-Cdk7 antibody
See all Cdk7 primary antibodies
DescriptionRabbit polyclonal to Cdk7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 1-346 of the Human Cdk7 protein (P50613).
- WB: 293T, A431, H1299, HeLa, HepG2, Molt-4, and Raji whole cell extracts. IHC-P: Human Breast ca.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa nuclear extract lysate (ab14655)
- HepG2 nuclear extract lysate (ab14660)
- HeLa nuclear extract lysate (ab150036)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- A431 whole cell lysate (ab30132)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab137716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
- Information by UniProt
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
All lanes : Anti-Cdk7 antibody (ab137716) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Hela whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human Breast ca tissue labelling Cdk7 with ab137716 at 1/250.
ab137716 has not yet been referenced specifically in any publications.