Recombinant Anti-Cdk7 antibody [BL-80-3D4] (ab243863)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cdk7 antibody [BL-80-3D4]
See all Cdk7 primary antibodies -
Description
Rabbit monoclonal [BL-80-3D4] to Cdk7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdk7 aa 300-346 (C terminal). The exact sequence is proprietary. Gene ID 1022.
Database link: P50613 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-80-3D4 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab243863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/50 - 1/200. | |
| IP | Use at an assay dependent concentration. Use 20µl/0.5mg lysate. |
|
| IHC-P | 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. |
Target
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Function
Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1022 Human
- Omim: 601955 Human
- SwissProt: P50613 Human
- Unigene: 184298 Human
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Alternative names
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
see all
Images
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![Immunocytochemistry - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)](https://www.abcam.com/ps/products/243/ab243863/Images/ab243863-334248-anti-cdk7-antibody-bl-80-3d4-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)Formaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling brd4 using ab243863 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)](https://www.abcam.com/ps/products/243/ab243863/Images/ab243863-334247-anti-cdk7-antibody-bl-80-3d4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for cdk7 using ab243863 at 1/30 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining. -
![Immunoprecipitation - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)](https://www.abcam.com/ps/products/243/ab243863/Images/ab243863-334246-anti-cdk7-antibody-bl-80-3d4-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)cdk7 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243863 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243863 at 1/1000 dilution.Lane 1: ab243863 IP in HeLa cell lysate.
Lane 2: Contol IgG in HeLa cell lysate.
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![Western blot - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)](https://www.abcam.com/ps/products/243/ab243863/Images/ab243863-334245-anti-cdk7-antibody-bl-80-3d4-western-blot.jpg)
Western blot - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)Western blot analysis using ab243863 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: Jurkat whole cell lysate (15 µg).
Lane 4: MCF-7 whole cell lysate (15 µg).
Lane 5: HepG2 whole cell lysate (15 µg).
Lane 6: A549 whole cell lysate (15 µg).
Lane 7: SW620 whole cell lysate (15 µg).
Lane 8: SK whole cell lysate (15 µg).
Lane 9: HCT 116 whole cell lysate (15 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
References
ab243863 has not yet been referenced specifically in any publications.
