Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-80-3D4] to Cdk7
- Suitable for: ICC, IP, IHC-P, WB
- Reacts with: Human
Product nameAnti-Cdk7 antibody [BL-80-3D4]
See all Cdk7 primary antibodies
DescriptionRabbit monoclonal [BL-80-3D4] to Cdk7
Tested applicationsSuitable for: ICC, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cdk7 aa 300-346 (C terminal). The exact sequence is proprietary. Gene ID 1022.
Database link: P50613
This product is sold under License from Bethyl Laboratories, Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/200.|
|IP||Use at an assay dependent concentration.
Use 20µl/0.5mg lysate.
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
- Information by UniProt
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
Formaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling brd4 using ab243863 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for cdk7 using ab243863 at 1/30 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
cdk7 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243863 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243863 at 1/1000 dilution.
Lane 1: ab243863 IP in HeLa cell lysate.
Lane 2: Contol IgG in HeLa cell lysate.
Western blot analysis using ab243863 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: Jurkat whole cell lysate (15 µg).
Lane 4: MCF-7 whole cell lysate (15 µg).
Lane 5: HepG2 whole cell lysate (15 µg).
Lane 6: A549 whole cell lysate (15 µg).
Lane 7: SW620 whole cell lysate (15 µg).
Lane 8: SK whole cell lysate (15 µg).
Lane 9: HCT 116 whole cell lysate (15 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243863 has not yet been referenced specifically in any publications.