Key features and details
- Mouse monoclonal [IMD-26] to Cdk7
- Suitable for: WB, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Cdk7 antibody [IMD-26]
See all Cdk7 primary antibodies
DescriptionMouse monoclonal [IMD-26] to Cdk7
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Human
Recombinant Human Cdk7.
- IHC-P: Human breast tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.001% Sodium azide
Constituents: 98% PBS, 0.2% BSA, 1.2% Sodium acetate
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab130001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Predicted molecular weight: 39 kDa.|
|IHC-P||Use a concentration of 0.5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 µg/ml.|
FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
- Information by UniProt
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
ab130001 has not yet been referenced specifically in any publications.