Recombinant
RabMAb

Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)

Overview

  • Product name
    Anti-Cdk7 (phospho T170) antibody [EPR6650(2)]
    See all Cdk7 primary antibodies
  • Description
    Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170)
  • Host species
    Rabbit
  • Specificity
    This antibody only detects CDK7 phosphorylated at threonine 170.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk7 (phospho T170).
    Database link: P50613

  • Positive control
    • WB: HeLa, A431, NIH/3T3 and C6 cell lysates and rat spleen tissue lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
      Phosphorylation of Thr-170 is required for activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 39 kDa protein kinase antibody
      • CAK antibody
      • CAK1 antibody
      • Cdk activating kinase antibody
      • CDK-activating kinase 1 antibody
      • CDK-activating kinase antibody
      • cdk7 antibody
      • CDK7_HUMAN antibody
      • CDKN7 antibody
      • Cell division protein kinase 7 antibody
      • Cyclin dependent kinase 7 antibody
      • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
      • Cyclin-dependent kinase 7 antibody
      • HCAK antibody
      • Homolog of Xenopus MO15 Cdk activating kinase antibody
      • Kinase subunit of CAK antibody
      • MO15 antibody
      • MO15, Xenopus, homolog of antibody
      • P39 Mo15 antibody
      • p39MO15 antibody
      • Serine threonine kinase Stk1 antibody
      • Serine/threonine protein kinase 1 antibody
      • Serine/threonine protein kinase MO15 antibody
      • STK1 antibody
      • TFIIH basal transcription factor complex kinase subunit antibody
      see all

    Images

    • All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + A431 cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)

      Lane 1 : C6 cell lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    References

    ab155976 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155976.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up