Product nameAnti-Cdk8 antibody
See all Cdk8 primary antibodies
DescriptionRabbit polyclonal to Cdk8
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Cdk8 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P49336
- WB: A549, H1299 and HCT 116 whole cell lysate. IHC-P: Mouse testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- cdk8 antibody
- CDK8 protein kinase antibody
- CDK8_HUMAN antibody
All lanes : Anti-Cdk8 antibody (ab272879) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Paraffin-embedded mouse testis tissue stained for CDK8 using ab272879 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272879 has not yet been referenced specifically in any publications.