Key features and details
- Rabbit polyclonal to Cdk9
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cdk9 antibody
See all Cdk9 primary antibodies
DescriptionRabbit polyclonal to Cdk9
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to to a region within amino acids 1-50 of Human Cdk9 (NP_001252.1).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab124261 was affinity purified using an epitope specific to Cdk9 immobilized on solid support .
Our Abpromise guarantee covers the use of ab124261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
Anti-Cdk9 antibody which recognizes a downstream epitope of Cdk9 was used at 1 µg/ml detecting Cdk9 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with ab124261 at 6 µg/mg of lysate.
Lane 2: IP with an antibody which recognizes an downstream epitope of Cdk9.
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab124261 has not yet been referenced specifically in any publications.