Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdk9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cdk9 (internal sequence). The exact sequence is proprietary.
    Database link: P50750

  • Positive control

    • WB: HL-60 and NIH/3T3 whole cell lysates. IHC-P: Rat adrenal gland tissue; Mouse brain tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Anti-Cdk9 antibody (ab227211) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Anti-Cdk9 antibody (ab227211) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded mouse brain tissue stained for Cdk9 using ab227211 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat adrenal gland tissue stained for Cdk9 using ab227211 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cdk9 (green) using ab227211 at 1/500 dilution in ICC/IF.

    Red: Phalloidin at 1/200 dilution.

References

ab227211 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227211.
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