Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdk9
  • Host species
    Rabbit
  • Specificity
    No reaction is observed against other related cyclin dependent kinases.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)

  • General notes
    cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.

Properties

Applications

Our Abpromise guarantee covers the use of ab6544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21378166
ELISA 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.

Target

  • Function
    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.

  • Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.

    The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.

References

This product has been referenced in:
  • Kuzmina A  et al. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology 11:51 (2014). WB . Read more (PubMed: 24985467) »
  • Zhang H  et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Application
ChIP
Sample
Mouse Cell lysate - whole cell (G1E cell line)
Negative control
IgG ChIP on regions
Specification
G1E cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30 min of EGS+3 min of formald
Positive control
CHD4 ChIP on KIT TSS and TAL1 TSS

Abcam user community

Verified customer

Submitted Aug 02 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% TritonX100 in PBS
Specification
HEK293T
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 15 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
35 µg
Specification
HeLa Cells
Gel Running Conditions
Reduced Denaturing (8 % SDS gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 04 2013

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount
0.5 µg
Specification
Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Dr. Kei Miyamoto

Verified customer

Submitted Oct 10 2012

Answer

Thank you very much for your interest in our products.

We have three antibodies which may interest you as they are all tested for both IP and WB.In addition,the homology between the two species is 100% for this protein, therefore,it seems very likely that those antibodies will react with zebrafish material.

The antibodies are the following:

https://www.abcam.com/index.html?datasheet=6544 (or use the following: https://www.abcam.com/index.html?datasheet=6544).

https://www.abcam.com/index.html?datasheet=10874 (or use the following: https://www.abcam.com/index.html?datasheet=10874).

https://www.abcam.com/index.html?datasheet=76320 (or use the following: https://www.abcam.com/index.html?datasheet=76320).

I can offer a discount off a future purchase if you buyone, two or allantibodies now, test it/them in zebrafish and submit feedback to us in the form of an Abreview for the tested antibody. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody (again for either one, two or all, depending of .

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testone or more of the antibodiesin zebrafish. I will then send a discount code. This code must be issued before purchasing one or more of the antibodies so please wait for my reply before ordering.

2. Purchase one or more of the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in zebrafish.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if one or more of the antibodies turns out to be unsuitable for zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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