Anti-Cdk9 antibody (ab6544)
Key features and details
- Rabbit polyclonal to Cdk9
- Suitable for: IHC-P, ELISA, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cdk9 antibody
See all Cdk9 primary antibodies -
Description
Rabbit polyclonal to Cdk9 -
Host species
Rabbit -
Specificity
No reaction is observed against other related cyclin dependent kinases. -
Tested applications
Suitable for: IHC-P, ELISA, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Cdk9. Multiple synthetic peptides corresponding to C-terminal and N-terminal domains.
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Positive control
- WB: human kidney lysate, PC3 lysate IHC: mouse tissue
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General notes
cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
Sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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Notes |
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IHC-P
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
Target
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Function
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
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Alternative names
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
Images
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All lanes : Anti-Cdk9 antibody (ab6544) at 1/1000 dilution
Lane 1 : Opal PreStain 11-245 kDa (MB-210-0500) at 10 µl
Lane 2 : Human kidney lysate at 5 µg
Lane 3 : PC3 lysate at 20 µg
Lane 4 : Blank well
Predicted band size: 43 kDa
Exposure time: 46 seconds -
Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab6544 has been referenced in 8 publications.
- Sithole N et al. DDX5 potentiates HIV-1 transcription as a co-factor of Tat. Retrovirology 17:6 (2020). PubMed: 32228614
- Cary DC et al. HIV Transcription is Independent of Mediator Kinases. AIDS Res Hum Retroviruses N/A:N/A (2019). PubMed: 31044597
- Shan J et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). PubMed: 27471030
- Kuzmina A et al. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology 11:51 (2014). WB . PubMed: 24985467
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). PubMed: 23898190
- Qi T et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . PubMed: 21378166
- Kruse U et al. Chemoproteomics-based kinome profiling and target deconvolution of clinical multi-kinase inhibitors in primary chronic lymphocytic leukemia cells. Leukemia : (2010). PubMed: 20944678
- Iankova I et al. Peroxisome proliferator-activated receptor gamma recruits the positive transcription elongation factor b complex to activate transcription and promote adipogenesis. Mol Endocrinol 20:1494-505 (2006). PubMed: 16484339