Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdk9
  • Host species
    Rabbit
  • Specificity
    No reaction is observed against other related cyclin dependent kinases.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)

  • General notes
    cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.

Properties

Applications

Our Abpromise guarantee covers the use of ab6544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21378166
ELISA 1/500 - 1/2000.
IHC-P 1/200 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.

Target

  • Function
    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.

  • Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.

    The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.

References

This product has been referenced in:
  • Shan J  et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). Read more (PubMed: 27471030) »
  • Kuzmina A  et al. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology 11:51 (2014). WB . Read more (PubMed: 24985467) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-5 of 5 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Application
ChIP
Sample
Mouse Cell lysate - whole cell (G1E cell line)
Negative control
IgG ChIP on regions
Specification
G1E cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30 min of EGS+3 min of formald
Positive control
CHD4 ChIP on KIT TSS and TAL1 TSS

Abcam user community

Verified customer

Submitted Aug 02 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% TritonX100 in PBS
Specification
HEK293T
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 15 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
35 µg
Specification
HeLa Cells
Gel Running Conditions
Reduced Denaturing (8 % SDS gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 04 2013

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount
0.5 µg
Specification
Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Dr. Kei Miyamoto

Verified customer

Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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