Overview

  • Product name

    Anti-Cdk9 antibody - C-terminal
    See all Cdk9 primary antibodies
  • Description

    Goat polyclonal to Cdk9 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cdk9 aa 345-359 (C terminal) (Cysteine residue). (NP_001252.1)
    Sequence:

    C-KGSQITQQSTNQSRN


    Database link: P50750

  • Positive control

    • WB: NIH/3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Anti-Cdk9 antibody - C-terminal (ab223576) at 0.5 µg/ml + NIH/3T3 (mouse embyro fibroblast cell line) nuclear cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 40-45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223576.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up