Name: Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free
Brand: RabMAb
SKU: ab236045

Product name

Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free
See all Cdk9 primary antibodies
Description

Rabbit monoclonal [EPR3119Y] to Cdk9 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IP, ICC/IF, WB, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Cdk9 aa 350-450 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HT-29, NIH/3T3, C6, HL-60, HeLa or 293 cell lysates. IHC-P: Human breast carcinoma, Human Bladder, mouse testis tissue. ICC/IF: HT-29 cells FC: HeLa cells IP: HeLa cells
General notes

The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF^®).
ab236045 is a PBS-only buffer format of ab76320. Please refer to ab76320 for recommended dilutions, protocols, and image data.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR3119Y
Isotype

IgG
Research areas

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa nuclear extract lysate (ab14655)

Our Abpromise guarantee covers the use of ab236045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
Flow Cyt		1/60.

Function

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Tissue specificity

Ubiquitous.
Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P50750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
see all
Alternative names
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
- Cdk 9 antibody
- Cdk9 antibody
- CDK9_HUMAN antibody
- Cell division cycle 2-like protein kinase 4 antibody
- Cell division protein kinase 9 antibody
- CTK1 antibody
- Cyclin dependent kinase 9 antibody
- Cyclin-dependent kinase 9 antibody
- PITALRE antibody
- Serine/threonine-protein kinase PITALRE antibody
- TAK antibody
- Tat associated kinase complex catalytic subunit antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Cdk9 with Purified ab76320 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdk9 with Purified ab76320 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

This image was made using ab76320 which is the same antibody as ab236045 with BSA and Azide
ab76320 (purified) at 1:20 dilution (0.5µg) immunoprecipitating Cdk9 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab76320 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76320 in HeLa whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)This image is courtesy of an Abreview submitted by Kirk McManus.

Unpurified ab76320 (1/200) staining Cdk9 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

Unpurified ab76320 showing positive staining in Cervical carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

Unpurified ab76320 showing positive staining in Colonic adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

Unpurified ab76320 showing positive staining in Lung adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76320 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

Unpurified ab76320 showing positive staining in Normal tonsil tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76320).

Datasheet

ab236045 has not yet been referenced specifically in any publications.

Publishing research using ab236045? Please let us know so that we can cite the reference in this datasheet.

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Anti-Cdk9 antibody [EPR3119Y] - BSA and Azide free (ab236045)

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