Anti-Cdk9 (phospho T186) antibody (ab79178)
Key features and details
- Rabbit polyclonal to Cdk9 (phospho T186)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Cdk9 (phospho T186) antibody
See all Cdk9 primary antibodies -
Description
Rabbit polyclonal to Cdk9 (phospho T186) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Cdk9 (phospho T186).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa. |
Target
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Function
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
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Alternative names
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab79178 has been referenced in 3 publications.
- Huang Y et al. Human cytomegalovirus RNA2.7 inhibits RNA polymerase II (Pol II) Serine-2 phosphorylation by reducing the interaction between Pol II and phosphorylated cyclin-dependent kinase 9 (pCDK9). Virol Sin 37:358-369 (2022). PubMed: 35537980
- Li J et al. CDK9 inhibitor CDKI-73 is synergetic lethal with PARP inhibitor olaparib in BRCA1 wide-type ovarian cancer. Am J Cancer Res 10:1140-1155 (2020). PubMed: 32368391
- Pinto DO et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006