Key features and details
- Rabbit polyclonal to Cdk9 (phospho T186)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cdk9 (phospho T186) antibody
See all Cdk9 primary antibodies
DescriptionRabbit polyclonal to Cdk9 (phospho T186)
Specificityab79178 detects endogenous levels of CDK9 only when phosphorylated at threonine 186.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthesized phosphopeptide derived from human Cdk9 around the phosphorylation site of threonine 186 (R-Y-TP-N-R).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 43 kDa.|
FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
ab79178 has been referenced in 1 publication.
- Pinto DO et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006