RecombinantRabMAb

Recombinant Anti-CDKAL1 antibody [EPR11621-26] (ab169531)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11621-26] to CDKAL1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDKAL1 antibody [EPR11621-26]
    See all CDKAL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11621-26] to CDKAL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CDKAL1 aa 1-200. The exact sequence is proprietary.
    Database link: Q5VV42

  • Positive control

    • HepG2, HeLa, Raji and MDA-MB-435 cell lysates; HeLa cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11621-26

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.

    • Tissue specificity

      Expressed in pancreatic islets.

    • Involvement in disease

      Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

    • Sequence similarities

      Belongs to the methylthiotransferase family. CDKAL1 subfamily.
      Contains 1 MTTase N-terminal domain.
      Contains 1 TRAM domain.

    • Cellular localization

      Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • CDK5 regulatory subunit associated protein 1 like 1 antibody
        • CDK5 regulatory subunit-associated protein 1-like 1 antibody
        • CDKAL_HUMAN antibody
        • CDKAL1 antibody
        • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
        • tRNA t 6 A37 methylthiotransferase antibody
        • tRNA-t(6)A37 methylthiotransferase antibody
        see all

      Images

      • Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        All lanes : Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : MDA-MB-435 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 65 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Immunofluorescent analysis of HeLa cells labeling CDKAL1 with ab169531 at 1/50 dilution. DAPI nuclear staining (blue).
      • Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

        Secondary
        HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

        Predicted band size: 65 kDa

      • Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Anti-CDKAL1 antibody [EPR11621-26] (ab169531)

      References (1)

      Publishing research using ab169531? Please let us know so that we can cite the reference in this datasheet.

      ab169531 has been referenced in 1 publication.

      • Take K  et al. CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively Regulates Adipocyte Differentiation through Activation of Wnt Signaling Pathway. Sci Rep 7:7326 (2017). PubMed: 28779110

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