Recombinant
RabMAb

Anti-CDKAL1 antibody [EPR11621-26] (ab169531)

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Overview

  • Product name
    Anti-CDKAL1 antibody [EPR11621-26]
    See all CDKAL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11621-26] to CDKAL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human CDKAL1 (UniProt Q5VV42).

  • Positive control
    • HepG2, HeLa, Raji and MDA-MB-435 cell lysates; HeLa cells; Immunoprecipitation pellet from HepG2 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11621-26
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
    • Tissue specificity
      Expressed in pancreatic islets.
    • Involvement in disease
      Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similarities
      Belongs to the methylthiotransferase family. CDKAL1 subfamily.
      Contains 1 MTTase N-terminal domain.
      Contains 1 TRAM domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
    • Information by UniProt
    • Database links
      • Alternative names
        • CDK5 regulatory subunit associated protein 1 like 1 antibody
        • CDK5 regulatory subunit-associated protein 1-like 1 antibody
        • CDKAL_HUMAN antibody
        • CDKAL1 antibody
        • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
        • tRNA t 6 A37 methylthiotransferase antibody
        • tRNA-t(6)A37 methylthiotransferase antibody
        see all

      Images

      • Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        All lanes : Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : MDA-MB-435 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 65 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Immunofluorescent analysis of HeLa cells labeling CDKAL1 with ab169531 at 1/50 dilution. DAPI nuclear staining (blue).
      • Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
        Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

        Secondary
        HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

        Predicted band size: 65 kDa

      References

      This product has been referenced in:
      • Take K  et al. CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively Regulates Adipocyte Differentiation through Activation of Wnt Signaling Pathway. Sci Rep 7:7326 (2017). Read more (PubMed: 28779110) »

      See 1 Publication for this product

      Publishing research using ab169531? Please let us know so that we can cite the reference in this datasheet.

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