Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CDKAL1 antibody [EPR11621-26]
See all CDKAL1 primary antibodies -
DescriptionRabbit monoclonal [EPR11621-26] to CDKAL1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to residues in Human CDKAL1 (UniProt Q5VV42).
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Positive control
- HepG2, HeLa, Raji and MDA-MB-435 cell lysates; HeLa cells; Immunoprecipitation pellet from HepG2 cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR11621-26
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab169531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 65 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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FunctionCatalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
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Tissue specificityExpressed in pancreatic islets.
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Involvement in diseaseGenetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
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Sequence similaritiesBelongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain. -
Cellular localizationEndoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 54901 Human
- Entrez Gene: 68916 Mouse
- Omim: 611259 Human
- SwissProt: Q5VV42 Human
- SwissProt: Q91WE6 Mouse
- Unigene: 657604 Human
- Unigene: 212227 Mouse
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Alternative names
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
see all
Images
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![Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)](https://www.abcam.com/ps/datasheet/images/169/ab169531/CDKAL1-Primary-antibodies-ab169531-1.jpg)
Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)All lanes : Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)](https://www.abcam.com/ps/datasheet/images/169/ab169531/CDKAL1-Primary-antibodies-ab169531-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)Immunofluorescent analysis of HeLa cells labeling CDKAL1 with ab169531 at 1/50 dilution. DAPI nuclear staining (blue). -
![Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)](https://www.abcam.com/ps/datasheet/images/169/ab169531/CDKAL1-Primary-antibodies-ab169531-3.jpg)
Western blot - Anti-CDKAL1 antibody [EPR11621-26] (ab169531)Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 65 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Take K et al. CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively Regulates Adipocyte Differentiation through Activation of Wnt Signaling Pathway. Sci Rep 7:7326 (2017). Read more (PubMed: 28779110) »
