Recombinant
RabMAb

Recombinant Anti-CDKAL1 antibody [EPR11621] (ab168390)

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Overview

  • Product name

    Anti-CDKAL1 antibody [EPR11621]
    See all CDKAL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11621] to CDKAL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CDKAL1 aa 1-200. The exact sequence is proprietary.
    Database link: Q5VV42

  • Positive control

    • HepG2, Raji, MDA-MB-435 and HeLa cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11621

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.

    • Tissue specificity

      Expressed in pancreatic islets.

    • Involvement in disease

      Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

    • Sequence similarities

      Belongs to the methylthiotransferase family. CDKAL1 subfamily.
      Contains 1 MTTase N-terminal domain.
      Contains 1 TRAM domain.

    • Cellular localization

      Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • CDK5 regulatory subunit associated protein 1 like 1 antibody
        • CDK5 regulatory subunit-associated protein 1-like 1 antibody
        • CDKAL_HUMAN antibody
        • CDKAL1 antibody
        • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
        • tRNA t 6 A37 methylthiotransferase antibody
        • tRNA-t(6)A37 methylthiotransferase antibody
        see all

      Images

      • Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)
        Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)
        All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : Raji cell lysate
        Lane 3 : MDA-MB-435 cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 65 kDa

      • Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)
        Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)
        Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      References

      ab168390 has not yet been referenced specifically in any publications.

      Publishing research using ab168390? Please let us know so that we can cite the reference in this datasheet.

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