Product nameAnti-CDKAL1 antibody [EPR11621]
See all CDKAL1 primary antibodies
DescriptionRabbit monoclonal [EPR11621] to CDKAL1
Tested applicationsSuitable for: WB, ICC, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment, corresponding to residues in Human CDKAL1 (UniProt: Q5VV42).
- HepG2, Raji, MDA-MB-435 and HeLa cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab168390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 65 kDa.|
|ICC||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionCatalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Tissue specificityExpressed in pancreatic islets.
Involvement in diseaseGenetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesBelongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain.
Cellular localizationEndoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
- Information by UniProt
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa
Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab168390 has not yet been referenced specifically in any publications.