Recombinant Anti-CDKAL1 antibody [EPR11621] (ab168390)
![Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)](https://www.abcam.com/ps/products/168/ab168390/Images/CDKAL1-Primary-antibodies-ab168390-1.jpg "Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11621] to CDKAL1
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-CDKAL1 antibody [EPR11621]
See all CDKAL1 primary antibodies -
Description
Rabbit monoclonal [EPR11621] to CDKAL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Recombinant fragment within Human CDKAL1 aa 1-200. The exact sequence is proprietary.
Database link: Q5VV42 -
Positive control
- HepG2, Raji, MDA-MB-435 and HeLa cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11621 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
|
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 65 kDa. |
|
IP
1/10 - 1/100. |
Target
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Function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. -
Tissue specificity
Expressed in pancreatic islets. -
Involvement in disease
Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain. -
Cellular localization
Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 54901 Human
- Entrez Gene: 68916 Mouse
- Omim: 611259 Human
- SwissProt: Q5VV42 Human
- SwissProt: Q91WE6 Mouse
- Unigene: 657604 Human
- Unigene: 212227 Mouse
-
Alternative names
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
see all
Images
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Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)](https://www.abcam.com/ps/products/168/ab168390/Images/CDKAL1-Primary-antibodies-ab168390-2.jpg)
Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Anti-CDKAL1 antibody [EPR11621] (ab168390)](https://www.abcam.com/ps/products/168/ab168390/Images/ab168390-3-benefits-of-recombinant-antibodies.png)
Anti-CDKAL1 antibody [EPR11621] (ab168390)
Protocols
Datasheets and documents
References (0)
ab168390 has not yet been referenced specifically in any publications.
