Recombinant
Anti-CDKAL1 antibody [EPR11621] (ab168390)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CDKAL1 antibody [EPR11621]
See all CDKAL1 primary antibodies -
DescriptionRabbit monoclonal [EPR11621] to CDKAL1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment, corresponding to residues in Human CDKAL1 (UniProt: Q5VV42).
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Positive control
- HepG2, Raji, MDA-MB-435 and HeLa cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11621
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab168390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 65 kDa. | |
| ICC | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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FunctionCatalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
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Tissue specificityExpressed in pancreatic islets.
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Involvement in diseaseGenetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
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Sequence similaritiesBelongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain. -
Cellular localizationEndoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 54901 Human
- Entrez Gene: 68916 Mouse
- Omim: 611259 Human
- SwissProt: Q5VV42 Human
- SwissProt: Q91WE6 Mouse
- Unigene: 657604 Human
- Unigene: 212227 Mouse
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Alternative names
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
see all
Images
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![Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)](https://www.abcam.com/ps/datasheet/images/168/ab168390/CDKAL1-Primary-antibodies-ab168390-1.jpg)
Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)](https://www.abcam.com/ps/datasheet/images/168/ab168390/CDKAL1-Primary-antibodies-ab168390-2.jpg)
Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Datasheets and documents
Protocols
References
ab168390 has not yet been referenced specifically in any publications.
