Recombinant
RabMAb

Anti-CDKAL1 antibody [EPR11621] (ab168390)

Overview

  • Product name
    Anti-CDKAL1 antibody [EPR11621]
    See all CDKAL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11621] to CDKAL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to residues in Human CDKAL1 (UniProt: Q5VV42).

  • Positive control
    • HepG2, Raji, MDA-MB-435 and HeLa cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11621
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
    • Tissue specificity
      Expressed in pancreatic islets.
    • Involvement in disease
      Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similarities
      Belongs to the methylthiotransferase family. CDKAL1 subfamily.
      Contains 1 MTTase N-terminal domain.
      Contains 1 TRAM domain.
    • Cellular localization
      Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDK5 regulatory subunit associated protein 1 like 1 antibody
      • CDK5 regulatory subunit-associated protein 1-like 1 antibody
      • CDKAL_HUMAN antibody
      • CDKAL1 antibody
      • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
      • tRNA t 6 A37 methylthiotransferase antibody
      • tRNA-t(6)A37 methylthiotransferase antibody
      see all

    Images

    • All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : MDA-MB-435 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    • Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab168390 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab168390.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up