- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-CDKL5 antibody
See all CDKL5 primary antibodies -
DescriptionRabbit polyclonal to CDKL5
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Host speciesRabbit
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SpecificitySwissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
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Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human CDKL5.
(Peptide available as ab25756.)
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Positive control
- adult mouse brain
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab22453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa). | |
| IHC-P | 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-FoFr | 1/300. |
Target
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FunctionMediates phosphorylation of MECP2.
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Tissue specificityExpressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.
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Involvement in diseaseNote=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).
Defects in CDKL5 are a cause of epileptic encephalopathy early infantile type 2 (EIEE2) [MIM:300672]; also known as atypical CDKL5-related Rett syndrome. EIEE2 is a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. -
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 6792 Human
- Entrez Gene: 382253 Mouse
- Omim: 300203 Human
- SwissProt: O76039 Human
- SwissProt: Q3UTQ8 Mouse
- Unigene: 659851 Human
- Unigene: 443717 Mouse
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Alternative names
- Cdkl5 antibody
- CDKL5_HUMAN antibody
- Cyclin dependent kinase 5 transcript antibody
see all
Images
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Western blot - Anti-CDKL5 antibody (ab22453)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomAll lanes : Anti-CDKL5 antibody (ab22453) at 3 µg/ml
Lane 1 : mouse cortex
Lane 2 : mouse cortex withHuman CDKL5 peptide (ab25756) at 2 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 105 kDa
Observed band size: 120,200 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 minutes
Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKL5 antibody (ab22453)Image courtesy of Human Protein Atlas
ab22453 staining nuclear CDKL5 in Human appendix. The paraffin embedded human skin tissue was incubated with ab22453 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab22453 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CDKL5 antibody (ab22453)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, FranceIHC-FoFr image of CDKL5 staining using ab22453(1:300) on Mouse hippocampus sections. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
References
This product has been referenced in:
- Wang IT et al. Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice. Proc Natl Acad Sci U S A : (2012). WB . Read more (PubMed: 23236174) »
- Nemos C et al. Mutational spectrum of CDKL5 in early-onset encephalopathies: a study of a large collection of French patients and review of the literature. Clin Genet 76:357-71 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19793311) »
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