Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
Key features and details
- Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody
See all CDKN1A interacting zinc finger protein 1/CIZ1 primary antibodies -
Description
Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CDKN1A interacting zinc finger protein 1/CIZ1 aa 200-460.
Database link: NP_036259 -
Positive control
- A549, HeLa, HepG2, HCT116 IP: HeLa whole cell lysate. IHC-P: ES2 xenograft tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as CDKN1A interacting zinc finger protein 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
|
| WB |
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
|
|
| IHC-P |
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa. |
|
IHC-P
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended. |
Target
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Function
May regulate the subcellular localization of CIP/WAF1. -
Involvement in disease
Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk. -
Sequence similarities
Contains 1 matrin-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 25792 Human
- Omim: 116899 Human
- SwissProt: Q9ULV3 Human
- Unigene: 212395 Human
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Alternative names
- CDKI antibody
- CDKN1A-interacting zinc finger protein 1 antibody
- Cip1-interacting zinc finger protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)Immunohistochemical analysis of paraffin-embedded ES2 xenograft using ab102013 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)Paraformaldehyde-fixed HeLa(human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN1A interacting zinc finger protein 1/CIZ1 using ab102013 at 1/200 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)CDKN1A interacting zinc finger protein 1/CIZ1 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg of ab102013. Western blot was performed from the immunoprecipitate using ab102013 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab102013 IP in HeLa whole cell lysate.
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Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 91 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab102013 has been referenced in 3 publications.
- Zhou X et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). WB, IHC-P ; Human . PubMed: 29285191
- Gang X et al. Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells. Clin Exp Immunol N/A:N/A (2018). PubMed: 29920650
- Wang DQ et al. Ciz1 is a novel predictor of survival in human colon cancer. Exp Biol Med (Maywood) N/A:N/A (2014). PubMed: 24928862