Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

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Overview

  • Product name

    Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody
    See all CDKN1A interacting zinc finger protein 1/CIZ1 primary antibodies

  • Description

    Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human CDKN1A interacting zinc finger protein 1/CIZ1 aa 200-460.
    Database link: NP_036259

  • Positive control

    • A549, HeLa, HepG2, HCT116 IP: HeLa whole cell lysate. IHC-P: ES2 xenograft tissue. ICC/IF: HeLa cells.

  • General notes

     This product was previously labelled as CDKN1A interacting zinc finger protein 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab102013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.
IHC-P 1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.

Target

  • Function

    May regulate the subcellular localization of CIP/WAF1.

  • Involvement in disease

    Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk.

  • Sequence similarities

    Contains 1 matrin-type zinc finger.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CDKI antibody
      • CDKN1A-interacting zinc finger protein 1 antibody
      • Cip1-interacting zinc finger protein antibody
      • CIZ1 antibody
      • CIZ1_HUMAN antibody
      • cyclin dependent inhibitor 1A antibody
      • LSFR1 antibody
      • Melanoma differentiation-associated protein antibody
      • NP94 antibody
      • Nuclear protein NP94 antibody
      • OPHN3 antibody
      • P21 antibody
      • Zinc finger protein 356 antibody
      • ZNF356 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunohistochemical analysis of paraffin-embedded ES2 xenograft using ab102013 at 1/500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

      Paraformaldehyde-fixed HeLa(human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN1A interacting zinc finger protein 1/CIZ1 using ab102013 at 1/200 dilution (green) in ICC/IF.

      The nuclear counter stain is Hoechst 33342 (blue).

    • Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

      CDKN1A interacting zinc finger protein 1/CIZ1 was immunoprecipitated from  1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg of ab102013. Western blot was performed from the immunoprecipitate using ab102013 at 1/1000 dilution. 

      Lane 1: HeLa whole cell lysate 40 μg (Input).

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Lane 3: ab102013 IP in HeLa whole cell lysate.

    • Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 91 kDa

    References

    This product has been referenced in:

    • Zhou X  et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). WB, IHC-P ; Human . Read more (PubMed: 29285191) »
    • Gang X  et al. Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells. Clin Exp Immunol N/A:N/A (2018). Read more (PubMed: 29920650) »
    See all 3 Publications for this product

    Publishing research using ab102013? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Inquiry: I'm interested if there is a possibility to get small amount of antibodies for test purposes. If yes, what is the amount, concentration and price for that. We are interested for -Anti-CDKN1A interacting zinc finger protein 1 antibody (ab102013) -Anti-p53 antibody (ab1101) -Anti-Bcl2 antibody [100/D5] (ab692) -Anti-Bax antibody (ab7977) Thank you for answer Regards

    Read More
    Answer

    Thank you for contacting us. Unfortunately, as we carry over 70,000 products, it simply isn't feasible for us to keep small sample sizes of our products.

    However, we are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. More information on this offer can be found from the following link:

    https://www.abcam.com/collaboratordiscount

    In order to qualify for this offer you need to be issued with a testing discount code prior to the purchase of the antibody to be tested. Please do therefore let us know if you would like to participate in this scheme.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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