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    cdkn1a-interacting-zinc-finger-protein-1ciz1-antibody-ab102013.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Cip / Kip
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Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
  • Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
  • Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

Key features and details

  • Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody
    See all CDKN1A interacting zinc finger protein 1/CIZ1 primary antibodies
  • Description

    Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human CDKN1A interacting zinc finger protein 1/CIZ1 aa 200-460.
    Database link: NP_036259

  • Positive control

    • A549, HeLa, HepG2, HCT116 IP: HeLa whole cell lysate. IHC-P: ES2 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as CDKN1A interacting zinc finger protein 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab102013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
IHC-P
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.
Notes
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
IHC-P
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.

Target

  • Function

    May regulate the subcellular localization of CIP/WAF1.
  • Involvement in disease

    Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk.
  • Sequence similarities

    Contains 1 matrin-type zinc finger.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9ULV3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25792 Human
    • Omim: 116899 Human
    • SwissProt: Q9ULV3 Human
    • Unigene: 212395 Human
    • Alternative names

      • CDKI antibody
      • CDKN1A-interacting zinc finger protein 1 antibody
      • Cip1-interacting zinc finger protein antibody
      • CIZ1 antibody
      • CIZ1_HUMAN antibody
      • cyclin dependent inhibitor 1A antibody
      • LSFR1 antibody
      • Melanoma differentiation-associated protein antibody
      • NP94 antibody
      • Nuclear protein NP94 antibody
      • OPHN3 antibody
      • P21 antibody
      • Zinc finger protein 356 antibody
      • ZNF356 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunohistochemical analysis of paraffin-embedded ES2 xenograft using ab102013 at 1/500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunocytochemistry/ Immunofluorescence - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

      Paraformaldehyde-fixed HeLa(human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN1A interacting zinc finger protein 1/CIZ1 using ab102013 at 1/200 dilution (green) in ICC/IF.

      The nuclear counter stain is Hoechst 33342 (blue).

    • Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Immunoprecipitation - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)

      CDKN1A interacting zinc finger protein 1/CIZ1 was immunoprecipitated from  1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg of ab102013. Western blot was performed from the immunoprecipitate using ab102013 at 1/1000 dilution. 

      Lane 1: HeLa whole cell lysate 40 μg (Input).

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Lane 3: ab102013 IP in HeLa whole cell lysate.

    • Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Western blot - Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013)
      Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 91 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab102013? Please let us know so that we can cite the reference in this datasheet.

    ab102013 has been referenced in 3 publications.

    • Zhou X  et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). WB, IHC-P ; Human . PubMed: 29285191
    • Gang X  et al. Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells. Clin Exp Immunol N/A:N/A (2018). PubMed: 29920650
    • Wang DQ  et al. Ciz1 is a novel predictor of survival in human colon cancer. Exp Biol Med (Maywood) N/A:N/A (2014). PubMed: 24928862

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Inquiry: I'm interested if there is a possibility to get small amount of antibodies for test purposes. If yes, what is the amount, concentration and price for that. We are interested for -Anti-CDKN1A interacting zinc finger protein 1 antibody (ab102013) -Anti-p53 antibody (ab1101) -Anti-Bcl2 antibody [100/D5] (ab692) -Anti-Bax antibody (ab7977) Thank you for answer Regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you for contacting us. Unfortunately, as we carry over 70,000 products, it simply isn't feasible for us to keep small sample sizes of our products.

    However, we are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. More information on this offer can be found from the following link:

    https://www.abcam.com/collaboratordiscount

    In order to qualify for this offer you need to be issued with a testing discount code prior to the purchase of the antibody to be tested. Please do therefore let us know if you would like to participate in this scheme.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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