Key features and details
- Rabbit polyclonal to CDKN2AIP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDKN2AIP antibody
See all CDKN2AIP primary antibodies
DescriptionRabbit polyclonal to CDKN2AIP
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human testis tissue; RT 4, U 251 MG, Human plasma and liver lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionActivates p53/TP53 by CDKN2A-dependent and CDKN2A-independent pathways.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the CARF family.
Contains 1 DRBM (double-stranded RNA-binding) domain.
modificationsMay be ubiquitinated.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- Calcium response factor antibody
- CARF antibody
- CARF_HUMAN antibody
All lanes : Anti-CDKN2AIP antibody (ab122715) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122715 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122715, at 1/50 dilution, staining CDKN2AIP in paraffin-embedded Human testis tissue by Immunohistochemistry.
ab122715 has not yet been referenced specifically in any publications.