Key features and details
- Rabbit polyclonal to CDKN2AIP
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CDKN2AIP antibody
See all CDKN2AIP primary antibodies
DescriptionRabbit polyclonal to CDKN2AIP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human CDKN2AIP (NP_060102.1).
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140519 was affinity purified using an epitope specific to CDKN2AIP immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActivates p53/TP53 by CDKN2A-dependent and CDKN2A-independent pathways.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the CARF family.
Contains 1 DRBM (double-stranded RNA-binding) domain.
modificationsMay be ubiquitinated.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- Calcium response factor antibody
- CARF antibody
- CARF_HUMAN antibody
All lanes : Anti-CDKN2AIP antibody (ab140519) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds
Immunoprecipitation of CDKN2AIP from 293T whole cell lysate using ab140519 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated CDKN2AIP, ab140519 was used at 1 µg/ml.
Detection: chemiluminescence with an exposure time of 10 seconds.
ab140519 has been referenced in 1 publication.
- Chik F et al. Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine. Carcinogenesis 35:138-44 (2014). PubMed: 23985780