Overview

  • Product name

  • Description

    Rabbit polyclonal to CDKN2AIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human CDKN2AIP (NP_060102.1).

  • Positive control

    • 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab140519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 61 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-CDKN2AIP antibody (ab140519) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation of CDKN2AIP from 293T whole cell lysate using ab140519 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
    For blotting immunoprecipitated CDKN2AIP, ab140519 was used at 1 µg/ml.
    Detection: chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

  • Chik F  et al. Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine. Carcinogenesis 35:138-44 (2014). Read more (PubMed: 23985780) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293/3T3 J2)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
HEK293/3T3 J2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Simon Boudreault

Verified customer

Submitted Nov 09 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 J2)
Permeabilization
Yes - 0,15% Triton X-100, 5 min
Specification
NIH 3T3 J2
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Simon Boudreault

Verified customer

Submitted Nov 18 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up