Product nameAnti-CDKN2AIP antibody
See all CDKN2AIP primary antibodies
DescriptionRabbit polyclonal to CDKN2AIP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 1-50 of Human CDKN2AIP (NP_060102.1).
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140519 was affinity purified using an epitope specific to CDKN2AIP immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActivates p53/TP53 by CDKN2A-dependent and CDKN2A-independent pathways.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the CARF family.
Contains 1 DRBM (double-stranded RNA-binding) domain.
modificationsMay be ubiquitinated.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- Calcium response factor antibody
- CARF antibody
- CARF_HUMAN antibody
All lanes : Anti-CDKN2AIP antibody (ab140519) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds
Immunoprecipitation of CDKN2AIP from 293T whole cell lysate using ab140519 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated CDKN2AIP, ab140519 was used at 1 µg/ml.
Detection: chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Chik F et al. Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine. Carcinogenesis 35:138-44 (2014). Read more (PubMed: 23985780) »