Key features and details
- Rabbit polyclonal to CDKN2A/p14ARF
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDKN2A/p14ARF antibody
See all CDKN2A/p14ARF primary antibodies
DescriptionRabbit polyclonal to CDKN2A/p14ARF
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide derived from Human CDKN2A/p14ARF (UniProt Q8N726).
- Human placenta tissue; HeLa cells; HeLa cell extract.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Cellular localizationCytoplasmic and Nuclear
- ARF antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
All lanes : Anti-CDKN2A/p14ARF antibody (ab129755) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with immunizing peptide
Predicted band size: 17 kDa
ab129755, at 1/100 dilution, staining CDKN2A/p14ARF in HeLa cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunizing peptide.
ab129755, at 1/50 dilution, staining CDKN2A/p14ARF in paraffin-embedded Human placenta tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunizing peptide.
ab129755 has not yet been referenced specifically in any publications.