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Thanks for your response. To answer your questions, my samples are human neuroblastoma cell lines. The membranes were blocked in a 5% milk solution, and the antibody was diluted in 5% BSA. Secondary antibody was diluted 1:2500 in 5% BSA solution. I am needing an antibody specific to the C-terminus of p14ARF; if any of your other antibodies meet this requirement I'd be happy to try them. Thanks for you help,
Asked on Oct 18 2011
Thank you for your response and for sending the protocol information. We also have the goat polyclonal ab115663 that is specific for the C terminus- https://www.abcam.com/CDKN2A-p14ARF-antibody-ab115663.html Would you be interested in trying this antibody?
Answered on Oct 18 2011