Key features and details
- Mouse monoclonal [ARF 4C6/4] to CDKN2A/p14ARF
- Suitable for: Flow Cyt, ELISA, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-CDKN2A/p14ARF antibody [ARF 4C6/4]
See all CDKN2A/p14ARF primary antibodies
DescriptionMouse monoclonal [ARF 4C6/4] to CDKN2A/p14ARF
Tested applicationsSuitable for: Flow Cyt, ELISA, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
His-tagged p14ARF protein expressed in bacteria.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Clone numberARF 4C6/4
Our Abpromise guarantee covers the use of ab11048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Cellular localizationCytoplasmic and Nuclear
- ARF antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
ICC/IF image of ab11048 stained human MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11048, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2cells.
Overlay histogram showing HeLa cells stained with ab11048 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11048, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab11048 has been referenced in 5 publications.
- Lee CK et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). PubMed: 26212014
- Hamilton G et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget 5:6142-67 (2014). PubMed: 25071014
- Ma H & Pederson T Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway. Mol Biol Cell 18:2630-5 (2007). WB, ICC/IF ; Human . PubMed: 17494866
- Clark PA et al. Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2. Oncogene 21:4498-507 (2002). PubMed: 12085228
- Llanos S et al. Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus. Nat Cell Biol 3:445-52 (2001). PubMed: 11331871