Overview

  • Product name

    Anti-CDKN2A/p14ARF antibody - C-terminal
    See all CDKN2A/p14ARF primary antibodies
  • Description

    Rabbit polyclonal to CDKN2A/p14ARF - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CDKN2A/p14ARF (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q8N726

  • Positive control

    • WB: HeLa cell extract. IHC-P: Human esophageal carcinoma and colon cancer tissues. IP: HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IP 1/100 - 1/500.

Target

  • Relevance

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • ARF antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • INK4a antibody
    • Melanoma p16 inhibits CDK4 antibody
    • MLM antibody
    • MTS 1 antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • p16 INK4a antibody
    • p16INK4a antibody
    • p19 antibody
    • P19ARF antibody
    • TP16 antibody
    see all

Images

  • All lanes : Anti-CDKN2A/p14ARF antibody - C-terminal (ab227809) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : CDKN2A/p14ARF Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 14 kDa

  • Paraffin-embedded human colon cancer tissue stained for CDKN2A/p14ARF using ab227809  at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-CDKN2A/p14ARF antibody - C-terminal (ab227809) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa



    15% SDS-PAGE gel.

  • CDKN2A/p14ARF was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract using 5 μg of ab227809. Western blot analysis was performed using ab227809. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell extract.

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab227809 in HeLa whole cell extract.

     

  • Paraffin-embedded human esophageal carcinoma tissue stained for CDKN2A/p14ARF using ab227809 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-CDKN2A/p14ARF antibody - C-terminal (ab227809) at 1/500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : CDKN2A/p14ARF knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 14 kDa



    15% SDS-PAGE gel.

References

ab227809 has not yet been referenced specifically in any publications.

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