Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP271] to CDKN2A/p14ARF
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-CDKN2A/p14ARF antibody [SP271]
See all CDKN2A/p14ARF primary antibodies
DescriptionRabbit monoclonal [SP271] to CDKN2A/p14ARF
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CDKN2A/p14ARF aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q8N726
- IHC: Human cervical squamous cell carcinoma Tissue WB: Hela cell lysate ICC/IF: HeLa and MCF7 cells Flow Cyt: HEK293 and HeLa cells
FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
(Incubate for 1 hour at room temperature).
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 10 minutes at room temperature).
|Flow Cyt||1/400 - 1/420.
(Incubate for 30 minutes at 4°C).
RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Cellular localizationCytoplasmic and Nuclear
- ARF antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
"Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/500 (0.86 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control."
Anti-CDKN2A/p14ARF antibody [SP271] (ab216602) at 1/400 dilution + HeLa cell lysate
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/420 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma labeling CDKN2A/p14ARF with ab216602 at 1/100 dilution.
Flow cytometric analysis of HEK293 cells labeling CDKN2A/p14ARF with ab216602 at 1/400 dilution (green) compared to negative control rabbit IgG (blue) .
ab216602 has not yet been referenced specifically in any publications.