Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980)
Key features and details
- Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [1D7D2A1]
See all CDKN2A/p16INK4a primary antibodies -
Description
Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a -
Host species
Mouse -
Specificity
Expression levels of the CDKN2A/p16INK4a protein may vary with sample type. It’s barely expressed in normal tissue, and mostly expressed in some tumour tissues, such as cervical cancer, breast cancer and so on. Moreover, only expressed in some cell lines. Please see images for recommended positive controls.
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Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human CDKN2A/p16INK4a aa 1 to the C-terminus.
Database link: P42771 -
Positive control
- WB: Recombinant human CDKN2A protein; CDKN2A transfected HEK293 cells; HeLa, HepG2, HEK293 cell lysate. Flow Cytometry: HeLa cells. IHC-P: Human endometrial cancer and esophageal cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR286368-6 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D7D2A1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 17 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 17 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1029 Human
- Entrez Gene: 12578 Mouse
- Entrez Gene: 25163 Rat
- Omim: 600160 Human
- SwissProt: P42771 Human
- SwissProt: P51480 Mouse
- SwissProt: Q9R0Z3 Rat
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution + recombinant human CDKN2A protein
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.
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Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN2A using ab201980 at 1/200 dilution (green) compared with a negative control (red).
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All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution
Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CDKN2A (AA: 1-156)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues labeling CDKN2A using ab201980 at 1/200 dilution followed by DAB staining.
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All lanes : Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 17 kDa
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab201980 has been referenced in 16 publications.
- Liu W et al. ACSL1 promotes imatinib-induced chronic myeloid leukemia cell senescence by regulating SIRT1/p53/p21 pathway. Sci Rep 12:17990 (2022). PubMed: 36289272
- Gonzalez Rajal A et al. A non-genetic, cell cycle-dependent mechanism of platinum resistance in lung adenocarcinoma. Elife 10:N/A (2021). PubMed: 33983115
- Lifsics A et al. Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas. Viruses 13:N/A (2021). PubMed: 34072187
- Yang G et al. ZNRD1-AS1 knockdown alleviates malignant phenotype of retinoblastoma through miR-128-3p/BMI1 axis. Am J Transl Res 13:5866-5879 (2021). PubMed: 34306331
- Ma X et al. Oncosuppressive role of MicroRNA-205-3p in gastric cancer through inhibition of proliferation and induction of senescence: Oncosuppressive role of MicroRNA-205 in gastric cancer. Transl Oncol 14:101199 (2021). PubMed: 34388692