Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)
Key features and details
- Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [2D9A12]
See all CDKN2A/p16INK4a primary antibodies -
Description
Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human CDKN2A/p16INK4a.
Database link: P42771 -
Positive control
- IHC-P: Human brain tumor, ovarian carcinoma, cervix, skin and brain tissue. Rat liver tissue. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2D9A12 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P | (9) |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Recommended antigen retrieval:Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating.Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides.Cover the cap and heat for 15-20 minutes on electric cooker until water boils.Begin timing, only 2 minutes in high-pressureRemove the pressure cooker, Allow it to cool(Cool the slides at room temperature)Rinse the slides in PBS for 5 minutes |
Notes |
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Flow Cyt
1/50. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended antigen retrieval:Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating.Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides.Cover the cap and heat for 15-20 minutes on electric cooker until water boils.Begin timing, only 2 minutes in high-pressureRemove the pressure cooker, Allow it to cool(Cool the slides at room temperature)Rinse the slides in PBS for 5 minutes |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1029 Human
- Entrez Gene: 25163 Rat
- Omim: 600160 Human
- SwissProt: P42771 Human
- SwissProt: Q9R0Z3 Rat
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Zinc buffered formalin-fixed human cartilage tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/200 dilution for 15 hours at 4°C. A Goat-anti-mouse polyclonal-biotin was used at the secondary at a 1/200 dilution.
For further detail please see abreview.
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10% formalin-fixed rat kidney tissue stained for CDKN2A/p16INK4a using ab54210 at a 1/500 dilution for 16 hours at 4°C. A Cy3-conjugated donkey-anti-mouse was used at the secondary at a 1/200 dilution.
For further detail please see abreview.
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ab54210 staining CDKN2A/p16INK4a in human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000) for 20 minutes at 25°C. An undiluted HRP-conjugated mouse polymer was used as the secondary antibody.
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Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab54210 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
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ab54210 staining CDKN2A/p16INK4a in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. ab6785 (1/800) was used as the secondary antibody.
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ab54210 at a 1:500 staining CDKN2A/p16INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB) is shown.
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ab54210 at a 1:500 staining CDKN2A/p16INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.
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ab54210 at a 1:500 staining CDKN2A/p16INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (DAB staining) is shown.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (124)
ab54210 has been referenced in 124 publications.
- Safwan-Zaiter H et al. Dynamic Spatiotemporal Expression Pattern of the Senescence-Associated Factor p16Ink4a in Development and Aging. Cells 11:N/A (2022). PubMed: 35159350
- Liu W et al. Chemopreventive efficacy of salvianolic acid B phospholipid complex loaded nanoparticles against experimental oral carcinogenesis: implication of sustained drug release. Ann Transl Med 10:244 (2022). PubMed: 35402586
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Miyamoto S et al. Validation of a Patient-Derived Xenograft Model for Cervical Cancer Based on Genomic and Phenotypic Characterization. Cancers (Basel) 14:N/A (2022). PubMed: 35740635
- Scotece M et al. mtDNA variability determines spontaneous joint aging damage in a conplastic mouse model. Aging (Albany NY) 14:5966-5983 (2022). PubMed: 35779570