For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Will ab54210 work in ICC-IF. Has this been tested?
For what reason were HeLa cells chosen to test in flow cytometry? We are not sure they express this protein. Can HeLa really be used as a positive control?
Asked on Mar 14 2012
Thank you for your telephone enquiry yesterday afternoon.
As discussed on the phone, I am sorry to confirm that to our knowledge, ab54210 has not been testedin ICC-IF. All testedand guaranteedapplications and species are listed on the online datasheets, and these are updated as soon as we receive any new information.
I can offer a discount off a future purchase if you buy ab54210 now, test it in ICC-IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab54210 in ICC-IF. I will then send a discount code. This code must be issued before purchasing ab54210 so please wait for my reply before ordering.
2. Purchase ab54210 either by phone, fax, or online (www.abcam.com).
3. Test it in ICC-IF.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab54210 turns out to be unsuitable for ICC-IF, you will still receive the discount on your next purchase after your Abreview has been submitted.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Regarding the positive control, I can confirm that HeLa cells would be a suitable positive control. these have been used successfully in flow cytometry with this antibody. I also notice that many other CDKN2A/p16INK4a available from Abcam and other suppliers are tested in HeLa.
There are many publications which confirm the expression of CDKN2A/p16INK4ain HeLa cells, for example:
p16INK4a Prevents Centrosome Dysfunction and Genomic Instability in Primary Cells
Kimberly M. McDermott et al
I hope this will be helpful to you. Please let me know if you have any questions about the testing discount offer and I would be happy to help you further.
Answered on Mar 14 2012