For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Madam, Sir, We recently conducted PCR Array analyses and found five potential targets involved in the response we are assessing. We now want to do Western Blot analyses to confirm the modulation of these five proteins in a lysate of human lung cancer cells. One such protein is interferon alpha 1 for which Abcam offer five different antibodies, i.e. ab 8317, 10077, 86316, 11408 and 37607. We are asking your help to identify the best product for our application. Moreover, we selected the following antibodies for four of these targets and would like you to confirm our choices : TIMP3 : Ab39184 Maspin : Ab95451 p16 : Ab16123 TIMP1 : Ab61224 Thank you in advance for your technical assistance in our product selection. Sincerely,
Asked on Sep 21 2011
Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the goat polyclonal antibody ab86316 unavailable to Canadian customers. The remaining 4 products are very similar. However, I would recommend : - the anti-Interferon alpha antibody clone [AE3] reference ab10077 (www.abcam.com/ab10077) or - the anti-Interferon alpha antibody clone [C10F5] reference ab8317 (www.abcam.com/ab8317). These two mouse monoclonal antibodies, protein A purified, are currently in stock, and their unit size is 250µg which makes them cheaper than the other anti-IFNA1. About the other targets, I agree with you, I would recommend - ab39184 for the detection of TIMP3, - ab61224 for TIMP1, - ab95451 (Mouse monoclonal) for Maspin. ab22354 (Rabbit polyclonal) is also a good choice, - ab16123 for CDKN2A/p16INK4a. ab54210 is also well characterised with 2 publications and 3 Abreviews (customer feedbacks). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Sep 22 2011