Application
ELISA
Sample
Human Recombinant protein (Purified recombinant p16 (overexpressed in HEK293))
Specification
Purified recombinant p16 (overexpressed in HEK293)
Type
Sandwich (Capture)
Blocking step
SuperBlock PBS Pierce #37580 as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10µg/mL
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
This antibody worked well in a direct ELISA, however was only satisfactory when used as a capture antibody in a p16 with ab108349.
Abcam user community
Verified customer
Submitted Apr 28 2014