Product nameAnti-CDKN2A/p16INK4a antibody [EPR1473] (Phycoerythrin)
See all CDKN2A/p16INK4a primary antibodies
DescriptionRabbit monoclonal [EPR1473] to CDKN2A/p16INK4a (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P42771
- Flow Cyt: HEK293 cells ICC/IF: HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (ab108349)
- Anti-CDKN2A/p16INK4a antibody [EPR1473] - BSA and Azide free (ab186932)
- Anti-CDKN2A/p16INK4a antibody [EPR1473] (Alexa Fluor® 488) (ab192053)
- Anti-CDKN2A/p16INK4a antibody [EPR1473] (Alexa Fluor® 647) (ab192054)
- Anti-CDKN2A/p16INK4a antibody [EPR1473] (HRP) (ab192080)
Our Abpromise guarantee covers the use of ab209579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.
This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)
Cellular localizationCytoplasmic and Nuclear
FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
Overlay histogram showing HEK293 cells stained with ab209579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209579, 1/2500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HEK293 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab209579 staining CDKN2A/p16INK4a in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209579 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab209579 has not yet been referenced specifically in any publications.