Recombinant Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
![Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)](https://www.abcam.com/ps/products/232/ab232402/Images/ab232402-321189-anti-cdkn2a-p16ink4a-antibody-epr20418-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20418] to CDKN2A/p16INK4a - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free
See all CDKN2A/p16INK4a primary antibodies -
Description
Rabbit monoclonal [EPR20418] to CDKN2A/p16INK4a - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab232402 is the carrier-free version of ab211542.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20418 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 12578 Mouse
- SwissProt: P51480 Mouse
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MEF (mouse embryonic fibroblast cell line) cell line labeling CDKN2A/p16INK4a with ab211542 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).
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![Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)](https://www.abcam.com/ps/products/232/ab232402/Images/ab232402-321188-anti-cdkn2a-p16ink4a-antibody-epr20418-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)CDKN2A/p16INK4a was immunoprecipitated from 0.35 mg of MEF (mouse embryonic fibroblast cell line) whole cell lysate with ab211542 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211542 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: MEF whole cell lysate 10 μg (Input).
Lane 2: ab211542 IP in MEF whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211542 in MEF whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).
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![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)](https://www.abcam.com/ps/products/232/ab232402/Images/ab232402-308794-anti-cdkn2a-p16ink4a-antibody-epr20418-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)Immunofluorescent analysis of 100% methanol-fixed MEF (mouse embryonic fibroblast cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CDKN2A/p16INK4a with ab211542 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MEF cell line.
Negative control: NIH/3T3 (PMID: 15210712).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.
MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).
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![Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)](https://www.abcam.com/ps/products/232/ab232402/Images/ab232402-4-benefits-of-recombinant-antibodies.png)
Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232402 has not yet been referenced specifically in any publications.
