Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
Yes - Tween, Triton
Specification
Pancreatic cancer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
10 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detect
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This antibody works well for IHC-P. We also tested it in Multiplexed Ion Beam Imaging by Time Of Flight (MIBI-TOF) and for this application this antibody did not work. MIBI-TOF test was performed after conjugation of this antibody to metal: 150Nd. As shown in the picture- the metal conjugation damaged the binding activity of the antibody, which is likely the reason it did not work in MIBI-TOF.
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Submitted Mar 01 2022