Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)](https://www.abcam.com/ps/products/267/ab267833/Images/ab267833-414570-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-med.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)")
Key features and details
- Mouse monoclonal [JC2] to CDKN2A/p16INK4a
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [JC2]
See all CDKN2A/p16INK4a primary antibodies -
Description
Mouse monoclonal [JC2] to CDKN2A/p16INK4a -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. Purified.
Database link: P42771 -
Positive control
- IHC-P: Human uterine cervical squamous cell carcinoma, squamous intraepithelial (HSIL) lesions and tonsil tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.099% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
JC2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100.
Antigen retrieval: EDTA buffer pH 8.0. Tissue fixed in 10% formalin are suitable for use prior to paraffin embedding. Incubate with primary antibody for 30 mins at RT. |
| Notes |
|---|
|
IHC-P
1/50 - 1/100. Antigen retrieval: EDTA buffer pH 8.0. Tissue fixed in 10% formalin are suitable for use prior to paraffin embedding. Incubate with primary antibody for 30 mins at RT. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1029 Human
- Omim: 600160 Human
- SwissProt: P42771 Human
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
Formalin-fixed, parafin-embedded high-grade squamous intraepithelial (HSIL) lesions stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)](https://www.abcam.com/ps/products/267/ab267833/Images/ab267833-366272-anti-p16-antibody-jc2-immunohistochemistry-cervical-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)Formalin-fixed, parafin-embedded human uterine cervical squamous cell carcinoma tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)](https://www.abcam.com/ps/products/267/ab267833/Images/ab267833-414571-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-med.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)Formalin-fixed, parafin-embedded tonsil tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain. Scatter epithelial cells display a moderate to strong nuclear and cytoplasmic staining reaction.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)](https://www.abcam.com/ps/products/267/ab267833/Images/ab267833-414572-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-Highgrade-squamous-intraepithelial-HSIL-lesions-stained-with-p16-med.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)Formalin-fixed, parafin-embedded uterine cervix tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 mins at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain. No staining reaction is seen in the squamous epithelial cell.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab267833 has been referenced in 1 publication.
- Perfecto-Avalos Y et al. Discriminant analysis and machine learning approach for evaluating and improving the performance of immunohistochemical algorithms for COO classification of DLBCL. J Transl Med 17:198 (2019). PubMed: 31185999
