Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)

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Key features and details

  • Mouse monoclonal [JC2] to CDKN2A/p16INK4a
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody [JC2]
    See all CDKN2A/p16INK4a primary antibodies

  • Description

    Mouse monoclonal [JC2] to CDKN2A/p16INK4a

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. Purified.
    Database link: P42771

  • Positive control

    • IHC-P: Human uterine cervical squamous cell carcinoma, squamous intraepithelial (HSIL) lesions and tonsil tissues

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab267833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Antigen retrieval: EDTA buffer pH 8.0. Tissue fixed in 10% formalin are suitable for use prior to paraffin embedding.

Incubate with primary antibody for 30 mins at RT.

Target

  • Cellular localization

    Cytoplasmic and Nuclear

  • Database links

    • Form

      There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.

    • Alternative names

      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)

      Formalin-fixed, parafin-embedded high-grade squamous intraepithelial (HSIL) lesions stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)

      Formalin-fixed, parafin-embedded human uterine cervical squamous cell carcinoma tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature.  Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)

      Formalin-fixed, parafin-embedded tonsil tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 minutes at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain. Scatter epithelial cells display a moderate to strong nuclear and cytoplasmic staining reaction.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [JC2] (ab267833)

      Formalin-fixed, parafin-embedded uterine cervix tissue stained for CDKN2A/p16INK4a using ab267833 at 1/50 dilution for 30 mins at room temperature. Antigen retrieval was performed using TEDTA buffer pH 8 and blue Hematoxylin was used as a counter stain. No staining reaction is seen in the squamous epithelial cell. 

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab267833? Please let us know so that we can cite the reference in this datasheet.

    ab267833 has been referenced in 1 publication.

    • Perfecto-Avalos Y  et al. Discriminant analysis and machine learning approach for evaluating and improving the performance of immunohistochemical algorithms for COO classification of DLBCL. J Transl Med 17:198 (2019). PubMed: 31185999

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