Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody - N-terminal
    See all CDKN2A/p16INK4a primary antibodies
  • Description

    Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human CDKN2A/p16INK4a (N terminal). 19 amino acid peptide.
    Database link: P42771

  • Positive control

    • Human colon tissues. Human colon cells. Mouse colon tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Form

    There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • Alternative names

    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Images

  • Lane 1 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 1 µg/ml
    Lane 2 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 2 µg/ml

    All lanes : mouse colon tissue lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CDKN2A/p16INK4a with ab189034 at 5 µg/ml. Heat mediated antigen retrieval was performed.

  • Immunofuorescent analysis of human colon cells labeling CDKN2A using ab189034 at 20 µg/ml.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human colon tissue labeling CDKN2A using ab189034 at 5 µg/ml.

References

This product has been referenced in:

  • Wan Y  et al. Knockout of a-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes. Lab Invest N/A:N/A (2019). Read more (PubMed: 30700848) »
  • Nguyen HQ  et al. Calpain-dependent Beclin1 cleavage stimulates senescence-associated cell death in HT22 hippocampal cells under the oxidative stress conditions. Neurosci Lett 701:106-111 (2019). Read more (PubMed: 30807795) »
See all 14 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Eyes)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Eyes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Xuan Du

Verified customer

Submitted Dec 24 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Archana Raman

Verified customer

Submitted Dec 08 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse chondrocytes)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Treatment
siRNA against CDKN2a
Specification
mouse chondrocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 08 2016

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