Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)
Key features and details
- Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody - N-terminal
See all CDKN2A/p16INK4a primary antibodies -
Description
Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal -
Host species
Rabbit -
Specificity
Expression levels of the CDKN2A/p16INK4a protein may vary with sample type. It’s barely expressed in normal tissue, and mostly expressed in some tumour tissues, such as cervical cancer, breast cancer and so on. Moreover, only expressed in some cell lines. Please see images for recommended positive controls.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human CDKN2A/p16INK4a (N terminal). 19 amino acid peptide.
Database link: P42771 -
Positive control
- Human colon tissues. Human colon cells. Mouse colon tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB | (2) |
Use a concentration of 1 - 2 µg/ml.
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| IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1029 Human
- Entrez Gene: 12578 Mouse
- Entrez Gene: 25163 Rat
- Omim: 600160 Human
- SwissProt: P42771 Human
- SwissProt: P51480 Mouse
- SwissProt: Q9R0Z3 Rat
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Western blot - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)Lane 1 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 1 µg/ml
Lane 2 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 2 µg/ml
All lanes : mouse colon tissue lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CDKN2A/p16INK4a with ab189034 at 5 µg/ml. Heat mediated antigen retrieval was performed.
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Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)Immunofuorescent analysis of human colon cells labeling CDKN2A using ab189034 at 20 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)Immunohistochemical analysis of formalin fixed, paraffin embedded human colon tissue labeling CDKN2A using ab189034 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab189034 has been referenced in 41 publications.
- Raffaele M et al. Mild exacerbation of obesity- and age-dependent liver disease progression by senolytic cocktail dasatinib?+?quercetin. Cell Commun Signal 19:44 (2021). PubMed: 33832488
- Miao J et al. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells. Physiol Rep 9:e14696 (2021). PubMed: 33463897
- Lin YF et al. Cellular senescence as a driver of cognitive decline triggered by chronic unpredictable stress. Neurobiol Stress 15:100341 (2021). PubMed: 34095365
- Lee HJ et al. Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging-related kidney injury. Aging Cell 20:e13407 (2021). PubMed: 34118180
- Gong W et al. Brahma-related gene-1 promotes tubular senescence and renal fibrosis through Wnt/ß-catenin/autophagy axis. Clin Sci (Lond) 135:1873-1895 (2021). PubMed: 34318888