Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse chondrocytes)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Treatment
siRNA against CDKN2a
Specification
mouse chondrocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL prime
Negative control
cells treated with siRNA
Exposure
10 second(s)
Bands
Specific: 16 kDa
Positive control
cells not treated with siRNA
Abcam user community
Verified customer
Submitted Aug 08 2016