Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Left ventricle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization
No
Specification
Left ventricle
Blocking step
Protein block serum-free as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA-PBS
Concentration
5 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Positive control
A) secondary antibody control for Alexa Fluor 488
B) DAPI channel, CDKN2A channel, and Combination of DAPI and CDKN2A
Pig left-ventricular tissue
C) DAPI
D) CDKN2A
E) Wheat-Germ-Agglutinin (WGA)
F) DAPI+CDKN2A+WGA
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Submitted Mar 04 2022