Recombinant Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)](https://www.abcam.com/ps/products/241/ab241543/Images/ab241543-1-anti-cdkn2ap16ink4a-antibody-pablo33-immunohistochemistry-wild-type-mouse-fibrosarcoma-and-p16-knockout-mouse-fibrosarcoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a
- Suitable for: IHC-P
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [PABLO33B]
See all CDKN2A/p16INK4a primary antibodies -
Description
Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Wild type mouse fibrosarcoma tissue. Mouse papilloma tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
PABLO33B -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/640.
|
| Notes |
|---|
|
IHC-P
1/640. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 12578 Mouse
- SwissProt: P51480 Mouse
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)The image was kindly provided by Spanish Nacional Cancer Research Centre (CNIO)
Immunohistochemical analysis of paraffin-embedded Wild type mouse fibrosarcoma and p16 knock-out mouse fibrosarcoma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 µg/mL).
Image A: Positive staining on the wild type mouse fibrosarcoma
Image B: No staining on the p16 knock-out mouse fibrosarcoma
The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)](https://www.abcam.com/ps/products/241/ab241543/Images/ab241543-2-anti-cdkn2ap16ink4a-antibody-pablo33-immunohistochemistry-mouse-papilloma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)The image was kindly provided by Spanish Nacional Cancer Research Centre (CNIO)Immunohistochemical analysis of paraffin-embedded mouse papilloma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 µg/mL). Positive staining on the mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
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![Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)](https://www.abcam.com/ps/products/241/ab241543/Images/ab241543-3-benefits-of-recombinant-antibodies.png)
Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241543 has not yet been referenced specifically in any publications.