For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cdkn2ap19arf-antibody-5-c3-ab26696.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cell Cycle Inhibitors Ink4
Share by email

Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (20)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
  • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
  • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

Key features and details

  • Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Suitable for: WB, IP, ICC/IF, IHC-FoFr
  • Reacts with: Mouse
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

HRP

You may also be interested in

Secondary
Product image
Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
Protein
Recombinant Human TWSG1/TSG protein (ab151919)
Primary
Product image
Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)

View more associated products

Overview

  • Product name

    Anti-CDKN2A/p19ARF antibody [5-C3]
    See all CDKN2A/p19ARF primary antibodies
  • Description

    Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.

  • Positive control

    • ICC/IF: MEF1 cells WB: MEF1 cell lysate, NIH3T3 cells
  • General notes

    The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    5-C3
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2b
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf

Associated products

  • Alternative Versions

    • Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)
    • HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab26696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-FoFr
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
1/1000.
IHC-FoFr
Use at an assay dependent concentration.

Target

  • Relevance

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 12578 Mouse
    • SwissProt: Q64364 Mouse
    • Alternative names

      • ARF antibody
      • CDKN2A antibody
      • Cyclin dependent kinase inhibitor 2A antibody
      • INK4 antibody
      • MTS1 antibody
      • P19 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

      ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5µg/ml and ab190573,  Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2µg/ml (shown in red). The secondary antibody (shown in green) was ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
    • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/ml + MEF-1 whole cell lysate at 20 µg

      Secondary
      Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Predicted band size: 17 kDa
      Observed band size: 19,85 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking buffer: 2% BSA

      Gel type: MES

    • Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

      Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
      Lane 2 : MEF total cell lysate

      Secondary
      All lanes : anti-rat IgG-HRP

      Predicted band size: 17 kDa
      Observed band size: 19 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
      Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (20)

    Publishing research using ab26696? Please let us know so that we can cite the reference in this datasheet.

    ab26696 has been referenced in 20 publications.

    • Sun L  et al. Nicotine triggers islet ß cell senescence to facilitate the progression of type 2 diabetes. Toxicology 441:152502 (2020). PubMed: 32473187
    • Strauss RP  et al. Loss of ARF/INK4A Promotes Liver Progenitor Cell Transformation Toward Tumorigenicity Supporting Their Role in Hepatocarcinogenesis. Gene Expr 20:39-52 (2020). PubMed: 32317048
    • Chu B  et al. ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway. Nat Cell Biol 21:579-591 (2019). PubMed: 30962574
    • Lázaro S  et al. Ablating all three retinoblastoma family members in mouse lung leads to neuroendocrine tumor formation. Oncotarget 8:4373-4386 (2017). PubMed: 27966456
    • Lee CK  et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). PubMed: 26212014
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-CDKN2A/p19ARF antibody [5-C3]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (cardiac tissues)
    Permeabilization
    Yes - 0.25% Triton X-100
    Specification
    cardiac tissues
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 22 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.