Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
- Datasheet
- References (15)
- Protocols
Overview
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Product name
Anti-CDKN2A/p19ARF antibody [5-C3]
See all CDKN2A/p19ARF primary antibodies -
Description
Rat monoclonal [5-C3] to CDKN2A/p19ARF -
Host species
Rat -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Hamster, Human -
Immunogen
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
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Positive control
- 3T3 cell lystate positive control.
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General notes
The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5-C3 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000. | |
| IHC-FoFr | Use a concentration of 5 µg/ml. |
Target
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Relevance
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
- Unigene: 4733 Mouse
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Alternative names
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696-37662-anti-cdkn2a-p19arf-antibody-5-c3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4). -
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696-37664-anti-cdkn2a-p19arf-antibody-5-c3-western-blot.jpg)
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
Lane 2 : MEF total cell lysate
Secondary
All lanes : anti-rat IgG-HRP
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696-37663-anti-cdkn2a-p19arf-antibody-5-c3-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
Protocols
References
This product has been referenced in:
- Lee CK et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). Read more (PubMed: 26212014) »
- Bartesaghi S et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »
