Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
- Datasheet
- References (15)
- Protocols
Overview
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Product nameAnti-CDKN2A/p19ARF antibody [5-C3]
See all CDKN2A/p19ARF primary antibodies -
DescriptionRat monoclonal [5-C3] to CDKN2A/p19ARF
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Host speciesRat
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Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-FoFrmore details
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Species reactivityReacts with: Mouse
Does not react with: Hamster, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse p19ARF.
Read Abcam's proprietary immunogen policy -
Positive control
- 3T3 cell lystate positive control.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% DL-Arginine -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone number5-C3
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MyelomaY3/Ag1.2.3
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IsotypeIgG2b
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000. | |
| IHC-FoFr | Use a concentration of 5 µg/ml. |
Target
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RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
- Unigene: 4733 Mouse
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Alternative names
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696_4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4). -
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696_1.jpg)
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
Lane 2 : MEF total cell lysate
Secondary
All lanes : anti-rat IgG-HRP
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.com/ps/products/26/ab26696/Images/ab26696_2.jpg)
Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
Protocols
References
This product has been referenced in:
- Lee CK et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). Read more (PubMed: 26212014) »
- Bartesaghi S et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »
