• Product name

    Anti-CDKN2A/p19ARF antibody [5-C3]
    See all CDKN2A/p19ARF primary antibodies
  • Description

    Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Host species

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.

  • Positive control

    • 3T3 cell lystate positive control.
  • General notes

    The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section. 

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.



Our Abpromise guarantee covers the use of ab26696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use a concentration of 5 µg/ml.


  • Relevance

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization

  • Database links

  • Alternative names

    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all


  • Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
  • All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
    Lane 2 : MEF total cell lysate

    All lanes : anti-rat IgG-HRP

    Predicted band size: 17 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.


This product has been referenced in:

  • Lee CK  et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). Read more (PubMed: 26212014) »
  • Bartesaghi S  et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »
See all 15 Publications for this product

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