Key features and details
- Rat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free
See all CDKN2A/p19ARF primary antibodies
DescriptionRat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse
Does not react with: Hamster, Human
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
- 3T3 cell lystate positive control.
This antibody clone is manufactured by Abcam.
This product is the PBS-only buffer version of ab26696.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
Our Abpromise guarantee covers the use of ab174939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
This ICC/IF data was generated using the same antibody clone in a different buffer formulation (ab26696).
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
ab174939 has not yet been referenced specifically in any publications.