Overview

  • Product name

    Anti-CDKN2A/p19ARF antibody [5-C3] (HRP)
    See all CDKN2A/p19ARF primary antibodies
  • Description

    Rat monoclonal [5-C3] to CDKN2A/p19ARF (HRP)
  • Host species

    Rat
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide within Mouse CDKN2A/p19ARF aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.

  • Positive control

    • WB: MEF-1 whole cell lystate.

Properties

Applications

Our Abpromise guarantee covers the use of ab202225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).

Target

  • Relevance

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all

Images

  • Anti-CDKN2A/p19ARF antibody [5-C3] (HRP) (ab202225) at 1/5000 dilution + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab202225 has not yet been referenced specifically in any publications.

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