Overview

  • Product name

    Anti-CDNF antibody [6G5]
    See all CDNF primary antibodies
  • Description

    Mouse monoclonal [6G5] to CDNF
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Human CDNF.
    Database link: Q49AH0

  • Positive control

    • WB: CDNF protein expressed into the supernatant of CHO cell culture medium. IHC-P: Human cerebral cortex tissue from Alzheimer’s disease patients. ICC/IF: CDNF expressing U2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab205551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/4000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100 - 1/300.

Target

  • Function

    Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.
  • Tissue specificity

    Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum.
  • Sequence similarities

    Belongs to the ARMET family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arginine rich mutated in early stage tumors like 1 antibody
    • ARMET L1 antibody
    • ARMET like protein 1 antibody
    • ARMET-like protein 1 antibody
    • ARMETL 1 antibody
    • ARMETL1 antibody
    • CDNF antibody
    • CDNF_HUMAN antibody
    • Cerebral dopamine neurotrophic factor antibody
    • Conserved dopamine neurotrophic factor antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer’s disease patients labeling CDNF with ab205551 at 5 µg/ml. Image on the right shows negative staining without primary antibody. Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium.

  • Anti-CDNF antibody [6G5] (ab205551) at 0.5 µg/ml + CDNF protein expressed into the supernatant of CHO cell culture medium

    Predicted band size: 21 kDa

  • Immunofluorescent analysis of U2OS cells labeling CDNF with ab205551 at 1 µg/ml. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody. Counterstained with DAPI (blue).

    Left panel: CDNF expressing U2OS cells.
    Right panel: Negative control (non-transfected U2OS cells).

References

ab205551 has not yet been referenced specifically in any publications.

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