Anti-CDON / Cdo antibody - N-terminal (ab227056)
Key features and details
- Rabbit polyclonal to CDON / Cdo - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CDON / Cdo antibody - N-terminal
See all CDON / Cdo primary antibodies -
Description
Rabbit polyclonal to CDON / Cdo - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CDON/ Cdo (N terminal). The exact sequence is proprietary.
Database link: Q4KMG0 -
Positive control
- WB: HeLa, HEK-293T, HepG2 and SH-SY5Y whole cell extracts. IHC-P: Human breast cancer tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 137,139 kDa. |
Target
-
Function
Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells. -
Involvement in disease
Holoprosencephaly 11 -
Sequence similarities
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 50937 Human
- Omim: 608707 Human
- SwissProt: Q4KMG0 Human
- Unigene: 38034 Human
-
Alternative names
- CDO antibody
- Cdon antibody
- CDON_HUMAN antibody
- Cell adhesion molecule-related/down-regulated by oncogenes antibody
Images
-
Western blot - Anti-CDON / Cdo antibody - N-terminal (ab227056)All lanes : Anti-CDON / Cdo antibody - N-terminal (ab227056) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 137,139 kDa5% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDON / Cdo antibody - N-terminal (ab227056)Paraffin-embedded human breast cancer tissue stained for CDON / Cdo using ab227056 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227056 has not yet been referenced specifically in any publications.
