Overview

  • Product name

  • Description

    Rabbit polyclonal to CDT1/DUP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human CDT1/DUP aa 1-230.
    Sequence:

    MEQRRVTDFF ARRRPGPPRI APPKLACRTP SPARPALRAP ASATSGSRKR ARPPAAPGRD QARPPARRRL RLSVDEVSSP STPEAPDIPA CPSPGQKIKK STPAAGQPPH LTSAQDQDTI SELASCLQRA RELGARVRAL KASAQDAGES CTPEAEGRPE EPCGEKAPAY QRFHALAQPG LPGLVLPYKY QVLAEMFRSM DTIVGMLHNR SETPTFAKVQ RGVQDMMRRR


    Database link: Q9H211

  • Positive control

    • NIH 3T3, U2OS and INS1 cell lysates.
  • General notes

     This product was previously labelled as CDT1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab183478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 60 kDa.
ICC/IF 1/2000.
IP Use at an assay dependent concentration.

Target

  • Function

    Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
  • Sequence similarities

    Belongs to the Cdt1 family.
  • Developmental stage

    Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
  • Domain

    The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
  • Post-translational
    modifications

    Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
    Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDT 1 antibody
    • cdt1 antibody
    • CDT1_HUMAN antibody
    • Chromatin licensing and DNA replication factor 1 antibody
    • DNA replication factor antibody
    • DNA replication factor Cdt1 antibody
    • Double parked antibody
    • Double parked Drosophila homolog of antibody
    • Double parked homolog antibody
    • DUP antibody
    • Retroviral integration site 1 antibody
    • Retroviral integration site 2 antibody
    • Retroviral integration site1 antibody
    • Retroviral integration site2 antibody
    • RIS 2 antibody
    • RIS2 antibody
    see all

Images

  • Immunofluorescent analysis of U2OS cells showing labeling of CDT1/DUP with ab183478 at 1/2000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.

    The cells were fixed in PBS containing 3% paraformaldehyde for 10 min, permeabilized for 2 min with cold methanol and washed 2 or 3 times with PBS. The cells were blocked in 2% BSA-PBS-Tween 20 0.2% (BSA-PBS-T) for 1h @ room temperature. The cells were incubated with ab183478 at 1/2000 in BSA-PBS-T overnight @ 4C. They were washed 3-4X with PBS-T (PBS-Tween 20 0.2%), incubated with secondary in BST-PBS-T 1 h @ room temperature, washed 3-4X with PBS-T and then washed 2X with PBS only.

  • All lanes : Anti-CDT1/DUP antibody (ab183478) at 1/2000 dilution

    Lane 1 : Human U2OS cell lysate
    Lane 2 : Rat INS1 cell lysate
    Lane 3 : Mouse NIH 3T3 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 60 kDa

  • Western blot analysis of immunoprecipitation of U2OS extracts

    Lane 1: Input.

    Lane 2: control serum.

    Lane 3: ab183478.

References

This product has been referenced in:

  • Ivanova EL  et al. Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development. Am J Hum Genet 101:428-440 (2017). Read more (PubMed: 28823707) »
  • Moreno A  et al. Unreplicated DNA remaining from unperturbed S phases passes through mitosis for resolution in daughter cells. Proc Natl Acad Sci U S A N/A:N/A (2016). WB ; Human . Read more (PubMed: 27516545) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - PBS with 1% Triton
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jul 31 2018

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