Product nameAnti-CDT1/DUP antibody
See all CDT1/DUP primary antibodies
DescriptionRabbit polyclonal to CDT1/DUP
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human CDT1/DUP aa 1-230.
MEQRRVTDFF ARRRPGPPRI APPKLACRTP SPARPALRAP ASATSGSRKR ARPPAAPGRD QARPPARRRL RLSVDEVSSP STPEAPDIPA CPSPGQKIKK STPAAGQPPH LTSAQDQDTI SELASCLQRA RELGARVRAL KASAQDAGES CTPEAEGRPE EPCGEKAPAY QRFHALAQPG LPGLVLPYKY QVLAEMFRSM DTIVGMLHNR SETPTFAKVQ RGVQDMMRRR
Database link: Q9H211
- NIH 3T3, U2OS and INS1 cell lysates.
This product was previously labelled as CDT1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab183478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 60 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionCooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
Sequence similaritiesBelongs to the Cdt1 family.
Developmental stagePresent during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
modificationsUbiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
- Information by UniProt
- CDT 1 antibody
- cdt1 antibody
- CDT1_HUMAN antibody
Immunofluorescent analysis of U2OS cells showing labeling of CDT1/DUP with ab183478 at 1/2000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.
The cells were fixed in PBS containing 3% paraformaldehyde for 10 min, permeabilized for 2 min with cold methanol and washed 2 or 3 times with PBS. The cells were blocked in 2% BSA-PBS-Tween 20 0.2% (BSA-PBS-T) for 1h @ room temperature. The cells were incubated with ab183478 at 1/2000 in BSA-PBS-T overnight @ 4C. They were washed 3-4X with PBS-T (PBS-Tween 20 0.2%), incubated with secondary in BST-PBS-T 1 h @ room temperature, washed 3-4X with PBS-T and then washed 2X with PBS only.
All lanes : Anti-CDT1/DUP antibody (ab183478) at 1/2000 dilution
Lane 1 : Human U2OS cell lysate
Lane 2 : Rat INS1 cell lysate
Lane 3 : Mouse NIH 3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 60 kDa
Western blot analysis of immunoprecipitation of U2OS extracts
Lane 1: Input.
Lane 2: control serum.
Lane 3: ab183478.
This product has been referenced in:
- Ivanova EL et al. Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development. Am J Hum Genet 101:428-440 (2017). Read more (PubMed: 28823707) »
- Moreno A et al. Unreplicated DNA remaining from unperturbed S phases passes through mitosis for resolution in daughter cells. Proc Natl Acad Sci U S A N/A:N/A (2016). WB ; Human . Read more (PubMed: 27516545) »