Overview

  • Product name

  • Description

    Rabbit polyclonal to CDT1/DUP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human CDT1/DUP (C terminal).
    Database link: NP_112190.1

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

     This product was previously labelled as CDT1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/2500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
  • Sequence similarities

    Belongs to the Cdt1 family.
  • Developmental stage

    Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
  • Domain

    The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
  • Post-translational
    modifications

    Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
    Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDT 1 antibody
    • cdt1 antibody
    • CDT1_HUMAN antibody
    • Chromatin licensing and DNA replication factor 1 antibody
    • DNA replication factor antibody
    • DNA replication factor Cdt1 antibody
    • Double parked antibody
    • Double parked Drosophila homolog of antibody
    • Double parked homolog antibody
    • DUP antibody
    • Retroviral integration site 1 antibody
    • Retroviral integration site 2 antibody
    • Retroviral integration site1 antibody
    • Retroviral integration site2 antibody
    • RIS 2 antibody
    • RIS2 antibody
    see all

Images

  • All lanes : Anti-CDT1/DUP antibody (ab70829) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 3 minutes


    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human islet cell carcinoma tissue labelling CDT1/DUP with ab70829 at 1/1000 (1µg/ml). Detection: DAB.

  • Detection of CDT1/DUP by Western Blot of Immunprecipitate.
    ab70829 at 1µg/ml staining CDT1/DUP in HeLa whole cell lysate immunoprecipitated using ab70829 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Secondary antibody anti-rabbit HRP (ab6721)
    Detection: Chemiluminescence with exposure time of 3 minutes.

References

This product has been referenced in:

  • Hernández-Pérez S  et al. USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication. Mol Oncol N/A:N/A (2016). WB ; Human . Read more (PubMed: 27296872) »
  • Grandy RA  et al. Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation. Mol Cell Biol 36:615-27 (2015). WB ; Human . Read more (PubMed: 26644406) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa S3 cervical cancer)
Gel Running Conditions
Reduced Denaturing (8% Tris-glycine SDS-PAGE)
Loading amount
50 µg
Treatment
double thymidine block and release
Specification
HeLa S3 cervical cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 05 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SAOS-2 OSTEOSARCOMA)
Permeabilization
Yes - PBS TRITON .25%
Specification
SAOS-2 OSTEOSARCOMA
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 03 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cells)
Permeabilization
Yes - Triton 0.5%
Specification
embryonic stem cells
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde

Mr. Shai Melcer

Verified customer

Submitted Nov 18 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Balb MK/ mouse keratinocyte / and 3T3 / and CHO)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
500000 cells
Specification
Balb MK/ mouse keratinocyte / and 3T3 / and CHO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Mr. Thinh Cao

Verified customer

Submitted May 07 2009

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