Overview

  • Product name

  • Description

    Rabbit polyclonal to CDT2/RAMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (His-tag) within Human CDT2/RAMP (C terminal). The exact sequence is proprietary.
    Database link: 51514

  • Positive control

    • HeLa whole cell extract and HeLa cells.
  • General notes

     This product was previously labelled as CDT2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab174385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/2000. Predicted molecular weight: 79 kDa.

Target

  • Function

    Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
  • Tissue specificity

    Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the WD repeat cdt2 family.
    Contains 7 WD repeats.
  • Developmental stage

    Expressed in all fetal tissues examined, included brain, lung, liver, and kidney.
  • Post-translational
    modifications

    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lethal(2) denticleless protein homolog antibody
    • CDW1 antibody
    • DCAF2 antibody
    • DDB1 and CUL4 associated factor 2 antibody
    • Ddb1- and Cul4-associated factor 2 antibody
    • Denticleless homolog antibody
    • Denticleless homolog (Drosophila) antibody
    • Denticleless protein homolog antibody
    • Dtl antibody
    • DTL_HUMAN antibody
    • L2DTL antibody
    • Lethal(2) denticleless protein homolog antibody
    • RA regulated nuclear matrix associated protein antibody
    • RAMP antibody
    • Retinoic acid regulated nuclear matrix associated protein antibody
    • Retinoic acid-regulated nuclear matrix-associated protein antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling CDT2/RAMP with ab174385  at 1/100 dilution (Red). The cells were stained for DNA with Hoechst 33258 (Blue). CDT2 protein is localized in nuclei at all cell stages.

  • Inhibition of CDT2/RAMP protein synthesis by CDT2/RAMP siRNA introduced into HeLa cells. siCont is control siRNA unrelated to CDT2/RAMP. siCDT2 is CDT2/RAMP specific siRNA.

    CDT2/RAMP is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).

  • Anti-CDT2/RAMP antibody (ab174385) at 1/2000 dilution + HeLa whole cell extract at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa

References

This product has been referenced in:

  • Nishitani H  et al. CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation. J Biol Chem 283:29045-52 (2008). WB, Fluorescent Cell Imaging ; Human . Read more (PubMed: 18703516) »
See 1 Publication for this product

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