Key features and details
- Rabbit polyclonal to CDT2/RAMP
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CDT2/RAMP antibody
See all CDT2/RAMP primary antibodies
DescriptionRabbit polyclonal to CDT2/RAMP
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-tag) within Human CDT2/RAMP (C terminal). The exact sequence is proprietary.
Database link: 51514
This product was previously labelled as CDT2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab174385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/2000. Predicted molecular weight: 79 kDa.|
FunctionSubstrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Tissue specificityExpressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the WD repeat cdt2 family.
Contains 7 WD repeats.
Developmental stageExpressed in all fetal tissues examined, included brain, lung, liver, and kidney.
modificationsUbiquitinated by the anaphase promoting complex/cyclosome (APC/C).
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
- Information by UniProt
- Lethal(2) denticleless protein homolog antibody
- CDW1 antibody
- DCAF2 antibody
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling CDT2/RAMP with ab174385 at 1/100 dilution (Red). The cells were stained for DNA with Hoechst 33258 (Blue). CDT2 protein is localized in nuclei at all cell stages.
Inhibition of CDT2/RAMP protein synthesis by CDT2/RAMP siRNA introduced into HeLa cells. siCont is control siRNA unrelated to CDT2/RAMP. siCDT2 is CDT2/RAMP specific siRNA.
CDT2/RAMP is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).
Anti-CDT2/RAMP antibody (ab174385) at 1/2000 dilution + HeLa whole cell extract at 20 µg
HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDa
ab174385 has been referenced in 1 publication.
- Nishitani H et al. CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation. J Biol Chem 283:29045-52 (2008). WB, Fluorescent Cell Imaging ; Human . PubMed: 18703516