Recombinant
RabMAb

Recombinant Anti-CDT2/RAMP antibody [EPR14978] (ab184548)

Overview

  • Product name

    Anti-CDT2/RAMP antibody [EPR14978]
    See all CDT2/RAMP primary antibodies
  • Description

    Rabbit monoclonal [EPR14978] to CDT2/RAMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CDT2/RAMP aa 50-200. The exact sequence is proprietary.
    Database link: Q9NZJ0

  • Positive control

    • WB: HEK-293, Jurkat and HeLa whole cell lysate (ab150035). IHC-P: Human thymus and testis tissue. ICC/IF: HeLa cells.
  • General notes

     

     

     This product was previously labelled as CDT2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 91 kDa (predicted molecular weight: 79 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt 1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
  • Tissue specificity

    Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the WD repeat cdt2 family.
    Contains 7 WD repeats.
  • Developmental stage

    Expressed in all fetal tissues examined, included brain, lung, liver, and kidney.
  • Post-translational
    modifications

    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lethal(2) denticleless protein homolog antibody
    • CDW1 antibody
    • DCAF2 antibody
    • DDB1 and CUL4 associated factor 2 antibody
    • Ddb1- and Cul4-associated factor 2 antibody
    • Denticleless homolog antibody
    • Denticleless homolog (Drosophila) antibody
    • Denticleless protein homolog antibody
    • Dtl antibody
    • DTL_HUMAN antibody
    • L2DTL antibody
    • Lethal(2) denticleless protein homolog antibody
    • RA regulated nuclear matrix associated protein antibody
    • RAMP antibody
    • Retinoic acid regulated nuclear matrix associated protein antibody
    • Retinoic acid-regulated nuclear matrix-associated protein antibody
    see all

Images

  • Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

  • Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/240 dilution (red) with negative control, Rabbit monoclonal IgG (green). Secondary antibody, Goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling CDT2/RAMP using ab184548 at a 1/100 dilution and Goat anti-rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution (red). Counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded sections of human testis tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded sections of human thymus tissue labeling CDT2/RAMP using ab184548 at a 1/100 dilution, counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • All lanes : Anti-CDT2/RAMP antibody [EPR14978] (ab184548) at 1/50000 dilution

    Lane 1 : HEK-293 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

References

ab184548 has not yet been referenced specifically in any publications.

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